FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13503646
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA29250.038.645099
TCTTCCG34050.032.9858788
CGTGTGC48400.023.1733131
CGTTGGA75200.016.6467741
GCGTCGG20000.013.967284140-144
AAACGGG80300.013.4491937
TCCGATC18400.013.06897410-14
ACGGGAT50800.013.0390119
GAAACGG83450.012.94152456
AACGGGA68600.012.4894518
GGAAACG89350.011.3617475
GTGCTCT113250.010.6799864
GTGTGCT120600.010.6513012
CCGATCT24350.010.526010510-14
TGTGCTC125800.09.7305063
GCTCTTC129450.09.4551286
CCGAGTA71350.09.4063032
TTCCGAT25150.09.38963310-14
CCCGAGT93400.08.8320151
AACGGGT21500.08.7067378
Produced by FastQC (version 0.11.5)