FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076621
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT5700.072.348441
GACGCTC13500.031.505892
TTCCGAT15600.028.610839
ACGCTCT19950.022.7567463
CTTCCGA20550.020.3179348
TCTTCCG23600.017.9976237
CGTTGGA57550.016.9713971
CGCTCTT26900.016.0575774
AAACGGG65100.014.7077357
GAAACGG68600.014.4820526
AACGGGA53750.013.527158
GGAAACG73550.013.5075775
ACGGGAT41300.012.5500619
CGCCATA7101.10562905E-511.2089131
ACCGCGC28950.010.4961471
AACGGGT17450.010.3135458
TCCGATC10450.09.23114210-14
CCCAAAG341900.08.8663341
CCAAAGT315800.08.5147172
CCGAGTA63650.08.4944722
Produced by FastQC (version 0.11.5)