FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076621
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA21100.031.3920369
TCTTCCG28450.023.7880978
CGTTGGA55850.020.6043221
CGTGTGC38450.018.2582781
AAACGGG65500.016.5977737
ACGGGAT39900.016.2399149
GAAACGG68350.015.4843496
AACGGGA54250.015.2620288
GGAAACG73100.014.2812025
AACGGGT17800.014.1567068
GCGTCGG14550.012.1726265140-144
TCCGATC12200.011.80267410-14
GCGCGAT15101.8189894E-1210.5145892
CCGATCT15300.09.9759610-14
ACGGGTT16651.6370905E-109.0806979
ACGGGTA11905.1482675E-79.0752479
GTTGGAA148350.09.0484042
GGCGCGA18551.4551915E-118.9736351
CGAAACG7250.0019446248.9375535
TTCCGAT16900.08.60547610-14
Produced by FastQC (version 0.11.5)