FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17337769
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA35950.030.8418039
TCTTCCG43600.026.9165258
CGTTGGA94900.019.9759581
CGTGTGC67150.017.3481181
ACGGGAT67400.015.275459
AAACGGG111500.015.1098057
GAAACGG116600.014.7576526
AACGGGA91500.014.2421448
GGAAACG123550.013.6361265
AACGGGT29500.012.4470398
TCCGATC22950.011.85822710-14
CGTCCGA5200.001263119911.07655056
CGAAACG10601.0468866E-69.5091135
CCGATCT30050.09.48770910-14
GCGTCGG17050.09.374312140-144
GTTGGAA246350.08.8750782
ACGGGTT30450.08.5120299
CCCAAAG549700.08.3978381
TTCCGAT30250.08.3777610-14
CCCAAGT245650.08.3651571
Produced by FastQC (version 0.11.5)