FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21998385
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24200.0103.446231
GACGCTC39200.063.8109322
TTCCGAT46350.053.2729039
ACGCTCT51150.049.447033
CTTCCGA56750.044.144378
CGCTCTT63900.038.0799644
TCTTCCG66450.038.0264637
CGTTGGA118000.017.7815651
TCCGATC35750.015.142755510-14
AAACGGG131500.015.1097457
ACGGGAT80800.014.9678669
AACGGGA107650.014.8457098
GAAACGG137500.014.1886276
CCGATCT42400.013.616693510-14
GGAAACG145800.012.9367295
GCTCTTC227400.011.3653995
AACGGGT32100.09.8675578
CGTATAA40700.09.0662931
CCCAAAG717850.08.9300511
AAGCGGC41750.08.8022243
Produced by FastQC (version 0.11.5)