FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21998385
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA49800.036.8660559
TCTTCCG58350.032.081038
CGTGTGC83050.024.3137441
CGTTGGA117700.018.570251
ACGGGAT81250.016.0387699
AACGGGA111550.015.4902178
AAACGGG135100.015.34804257
GAAACGG144100.014.6892356
GCGTCGG29300.014.202725140-144
GGAAACG152600.013.6351265
TCCGATC32700.013.16626410-14
CGACGCT6604.885158E-612.0624631
CCGATCT39100.011.63722510-14
GTGCTCT192600.010.6907174
GTGTGCT204500.010.5511972
CGCGATC30650.010.1017193
TTCCGAT40800.09.7053610-14
TGTGCTC218250.09.6995233
GCGCGAT31050.09.5289752
GCTCTTC215900.09.3372976
Produced by FastQC (version 0.11.5)