FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12704814
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT10750.085.9377441
GACGCTC20400.044.804892
TTCCGAT21800.042.2511639
ACGCTCT26650.035.1073463
CTTCCGA28250.032.859168
CGCTCTT34350.026.3998094
TCTTCCG36400.025.6996487
CGTTGGA68200.021.4387971
AAACGGG77050.018.0247767
AACGGGA63850.017.3558168
ACGGGAT46800.017.220969
GAAACGG84100.016.6849086
GGAAACG86100.015.7960685
AACGGGT20250.013.8587738
TCCGATC15450.012.94789310-14
CCGATCT19950.011.10940110-14
AAGCGGC22300.09.6820713
GTTGGAA175950.09.3669482
CCCAAAG416800.09.0928231
CCCAAGT180250.08.9994981
Produced by FastQC (version 0.11.5)