FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12704814
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA68250.019.359211
AACGGGA64300.017.455688
AAACGGG77200.016.962017
ACGGGAT49100.016.8515389
GAAACGG83250.015.5589956
GGAAACG87050.015.210465
CTTCCGA23400.014.7587179
TCTTCCG30750.011.4650048
AACGGGT21000.09.9357798
CCGAGTA69050.09.7978932
CCCAAAG391450.09.5510211
CCCGAGT95900.09.5382961
GTTGGAA169350.09.4773772
CGATCGG10350.09.198516140-144
GCGTCGG14950.08.973378140-144
CCCAAGT175150.08.8699771
CGAGTAG70900.08.8301133
CCAAAGT361250.08.8060872
CGGACGA11350.08.515167140-144
TCGGACG11900.08.485263140-144
Produced by FastQC (version 0.11.5)