FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14632429
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT14900.096.6643451
GACGCTC25750.055.899552
TTCCGAT28400.050.169199
ACGCTCT34650.041.9567833
CTTCCGA34150.041.932688
TCTTCCG40100.035.8901987
CGCTCTT44950.031.7031864
CGTTGGA77800.017.4843481
AACGGGA71550.015.9910778
AAACGGG88400.015.1408747
ACGGGAT55950.015.0479049
GAAACGG94450.014.2472126
TCCGATC21500.013.85624610-14
GGAAACG101250.013.1484435
CCGATCT27000.011.35349510-14
GCTCTTC149400.010.2113455
CCGAGTA82800.08.4313472
CCCAAAG471350.08.4262921
AGATCGG1606200.08.110387140-144
CGAGTAG80950.08.090563
Produced by FastQC (version 0.11.5)