FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14632429
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA28100.023.043919
CGTTGGA77050.018.7505341
TCTTCCG38400.017.237598
ACGGGAT54550.016.4867559
AACGGGA74450.014.8825038
AAACGGG90000.014.8692957
CGACGCT6001.0262738E-714.5198841
GAAACGG94400.013.5689616
CGTGTGC54350.012.8234481
GGAAACG100450.012.2502455
GCGTCGG20200.011.782977140-144
CCGAGTA75350.09.7429672
CCCAAAG445350.09.4060551
AACGGGT25600.09.2745738
CGATCGG12850.09.205199140-144
CCCGAGT105550.09.1480091
TCCGATC17550.09.01922110-14
CCAAAGT406400.08.8373322
CCCGAAT35650.08.7567331
CGAGTAG78350.08.7253583
Produced by FastQC (version 0.11.5)