FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15660947
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA89000.017.783391
ACGGGAT63100.016.3051229
AAACGGG98900.015.7863177
AACGGGA83400.015.4420298
GAAACGG106850.014.7489296
CTTCCGA28800.014.4894839
GGAAACG111450.013.8819115
GCGTCGG21050.012.060845140-144
CGACGCT6052.5778108E-512.0003171
TCTTCCG38550.011.1980988
AACGGGT25550.010.1374578
CCGAGTA84900.010.0882892
CCCGAGT114350.09.7776081
CGAGTAG85250.09.3695663
CCCAAAG478750.09.2809131
GTTGGAA211450.09.0542452
ACGGGTT26850.08.8427449
CCCAAGT214700.08.7244031
CCAAAGT443300.08.5888742
CGATCGG15400.08.5238905140-144
Produced by FastQC (version 0.11.5)