FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732583
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT13900.092.107431
GACGCTC25000.052.3976632
TTCCGAT28200.045.9310049
ACGCTCT34450.038.2332343
CTTCCGA36200.036.1780668
TCTTCCG43750.030.0992417
CGCTCTT43850.029.545344
CGTTGGA82650.019.011121
AACGGGA74950.016.129478
ACGGGAT58850.015.5288679
AAACGGG93750.015.2744067
GAAACGG98900.014.6245426
GGAAACG104100.014.1017055
TCCGATC21650.012.89602310-14
CCGATCT26850.011.20247510-14
GCTCTTC159050.08.958265
AACGGGT24100.08.9574998
CCCAAAG519350.08.7401981
GTTGGAA215450.08.5187182
CCAAAGT470050.08.29919052
Produced by FastQC (version 0.11.5)