FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21495105
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT16750.086.437161
GACGCTC35950.041.043072
TTCCGAT36950.040.1179439
CTTCCGA46250.033.1400958
ACGCTCT45650.032.0064963
TCTTCCG58650.025.3973397
CGCTCTT62200.023.9537184
CGTTGGA107400.015.309691
AACGGGA100100.012.9397038
ACGGGAT78650.012.534529
AAACGGG123650.012.4539047
GAAACGG136450.011.391116
TCCGATC28150.011.14527410-14
GGAAACG143250.010.5491295
CCGATCT35000.09.53965410-14
AGATCGG2170450.08.996099140-144
TAGATCG429800.08.802065140-144
GATCGGA2011350.08.795776140-144
CAGATCG449500.08.619843140-144
AAGATCG766500.08.344079140-144
Produced by FastQC (version 0.11.5)