FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592058
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT11650.069.314651
TTCCGAT25400.032.863489
GACGCTC25800.032.3608972
ACGCTCT30700.028.6022193
CTTCCGA34200.025.6698328
TCTTCCG43800.019.7149837
CGCTCTT44550.019.2261334
CGTTGGA85650.018.5164261
ACGGGAT60350.015.262379
AAACGGG98750.014.6469847
AACGGGA78650.014.5474788
GAAACGG107900.013.1381456
GGAAACG113400.012.24729255
AACGGGT27900.09.5430418
TCCGATC19800.09.23109810-14
AGATCGG1660450.09.038919140-144
TAGATCG327300.08.904984140-144
CAGATCG336650.08.851779140-144
CGATCGG9550.08.819754140-144
GATCGGA1539650.08.752074140-144
Produced by FastQC (version 0.11.5)