FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16592058
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA86000.018.2372931
AAACGGG104000.014.5971397
AACGGGA81350.014.5045728
GAAACGG112000.014.1996186
ACGGGAT61400.014.1786749
GGAAACG115600.013.1971595
GCGTCGG23300.012.011444140-144
AACGGGT29900.010.5876648
CGATCGG13550.010.327183140-144
AGATCGG1611150.08.817824140-144
CCGAGTA99800.08.7267812
GATCGGA1483800.08.497389140-144
TAGATCG317600.08.480339140-144
GTTGGAA225250.08.4679912
CAGATCG341100.08.310558140-144
CTTCCGA30700.08.20253859
CCCAAGT238100.08.1424851
ATCGGAA1374200.07.948956140-144
CCCGAGT140000.07.9353941
AAGATCG572600.07.8756614140-144
Produced by FastQC (version 0.11.5)