FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18349531
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT24450.0108.884151
GACGCTC38800.067.8897862
TTCCGAT41300.062.898989
ACGCTCT48900.054.4562763
CTTCCGA49200.052.6530888
TCTTCCG60250.042.996387
CGCTCTT64450.040.760034
CGTTGGA93450.018.914261
AACGGGA83550.016.8809178
ACGGGAT67500.016.523979
AAACGGG108250.016.0204817
TCCGATC34450.015.45698910-14
GAAACGG117100.014.8097116
GGAAACG119950.013.9781725
GCTCTTC197050.013.9502395
CCGATCT41400.013.87027110-14
AGATCGG1814850.08.927275140-144
TAGATCG350600.08.686357140-144
GATCGGA1687400.08.653105140-144
CAGATCG352550.08.58888140-144
Produced by FastQC (version 0.11.5)