FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18349531
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA35600.020.0082979
CGTTGGA98450.017.919971
ACGGGAT68900.015.1416679
AACGGGA95500.014.8418418
AAACGGG114650.014.6220017
TCTTCCG49300.014.4481828
GAAACGG119600.014.1394156
GGAAACG126650.013.1250685
GCGTCGG24600.011.199501140-144
CGATCGG15900.010.342087140-144
CGTGTGC73650.010.0548161
AACGGGT29400.09.054818
AGATCGG1758150.08.71877140-144
CAGATCG349950.08.565249140-144
GTTGGAA253800.08.564392
CGCGTCG11500.08.5292635140-144
GATCGGA1629250.08.470099140-144
TAGATCG344150.08.412015140-144
CCCAAAG568850.08.3341661
AAGATCG643000.08.194815140-144
Produced by FastQC (version 0.11.5)