FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11674465
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA47950.059.15949
TCTTCCG52200.054.480718
CGACGCT4700.050.8126641
CGTGTGC59950.047.200161
GTGCTCT123850.023.3101964
GTGTGCT130950.022.6497652
TGTGCTC134400.021.6977483
GCTCTTC140800.020.965126
CCGATCT40700.018.96317110-14
CGTTGGA64800.018.8741591
TCCGATC37750.018.4997310-14
ACGGGAT43500.015.3925259
AACGGGA58250.015.2028628
TGCTCTT196750.015.0764345
AAACGGG74200.014.7487717
TTCCGAT42550.014.3825389
GAAACGG81650.013.1385146
GGAAACG83400.012.5175155
CTCTTCC246200.012.42845257
GACGCTC14700.012.2729812
Produced by FastQC (version 0.11.5)