FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14101406
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT41750.0125.6211
GACGCTC52600.099.076372
TTCCGAT55800.094.307789
ACGCTCT59450.087.8736653
CTTCCGA62400.084.6790168
TCTTCCG69250.075.99157
CGCTCTT70300.073.731634
GCTCTTC175050.030.2686025
TCCGATC50750.021.24921610-14
CCGATCT55350.020.52373910-14
CGTTGGA73750.018.636841
CTCTTCC314900.017.1914546
ACGGGAT53650.015.29676159
AAACGGG83650.014.6302047
AACGGGA69850.014.1194748
GAAACGG90100.013.9823676
GGAAACG96500.013.1299185
CGATCTA15650.011.40786710-14
AACGGGT23450.09.516638
CGATCTG27150.09.49249410-14
Produced by FastQC (version 0.11.5)