FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267087
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT37800.0125.369171
GACGCTC50750.092.461982
TTCCGAT52400.089.848419
ACGCTCT57400.081.7189563
CTTCCGA59650.078.686658
TCTTCCG68300.068.4066857
CGCTCTT69550.066.557114
GCTCTTC177700.026.6169685
TCCGATC46450.021.01563310-14
CCGATCT52800.019.44258710-14
CGTTGGA73300.018.6551671
AACGGGA69200.015.7085028
AAACGGG87600.015.5321757
CTCTTCC316150.015.2565466
GAAACGG94450.014.4057026
ACGGGAT53000.014.2618989
GGAAACG96500.013.5776545
CGATCTA14300.012.38418910-14
CGATCTG26250.010.09222710-14
AACGGGT24450.09.7162578
Produced by FastQC (version 0.11.5)