FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14267087
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA44500.055.9798059
TCTTCCG51550.048.603318
CGTGTGC65450.038.479931
CGACGCT5201.8189894E-1220.8762551
GTGCTCT136150.019.3007324
TCCGATC30950.018.4700410-14
GTGTGCT147950.017.9486162
CGTTGGA72600.017.6441731
TGTGCTC151000.017.4528623
CCGATCT34500.017.19555310-14
GCTCTTC155450.016.6271956
ACGGGAT50450.015.555389
AAACGGG85700.014.3658267
AACGGGA70400.014.3176158
GAAACGG91800.013.8033746
TTCCGAT38550.013.18524710-14
GCGTCGG19500.012.5532255140-144
GGAAACG99150.012.4170585
TGCTCTT226150.012.065855
CGATCTA11450.09.9348110-14
Produced by FastQC (version 0.11.5)