FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12059968
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT9200.0103.042771
GACGCTC17100.055.6191862
TTCCGAT20950.046.0487829
ACGCTCT22650.041.9713133
CTTCCGA23350.041.932378
TCTTCCG29600.032.8351827
CGCTCTT31950.029.303494
CGTTGGA67750.018.905931
ACGGGAT48150.015.5501449
AAACGGG82450.015.1061177
GAAACGG84100.014.9861096
AACGGGA65950.014.8464128
GGAAACG86900.013.7573615
TCCGATC15400.013.46384710-14
CCGATCT17900.012.06606310-14
AACGGGT22200.010.377548
ACGGGTT20350.010.2596129
CAAAGCG45400.010.2013621
CCGAGTA55250.09.6504212
CCCGAAT27250.09.29470351
Produced by FastQC (version 0.11.5)