FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12059968
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA23850.040.7537469
TCTTCCG31350.031.6939458
CGTGTGC42150.024.113731
CGTTGGA71750.022.0021481
ACGGGAT49050.019.9628269
AACGGGA67200.018.2143828
AAACGGG84450.017.7336627
GAAACGG87300.017.4021536
GGAAACG87450.017.042975
TCCGATC15600.014.12276410-14
CCGATCT17450.012.95558610-14
AACGGGT22200.011.0270838
GTGCTCT98400.010.8293254
GTGTGCT108950.010.1143842
GTTGGAA177950.09.956632
GCGTCGG7700.09.911838140-144
TGTGCTC113600.09.8239953
TTCCGAT20900.09.57682110-14
GCTCTTC110800.09.2274846
CGCAAGT8851.2964451E-48.9596751
Produced by FastQC (version 0.11.5)