FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4860241
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT6550.0106.6915051
GACGCTC9250.074.627192
ACGCTCT11000.063.408373
TTCCGAT11150.063.201559
CTTCCGA12300.057.291288
CGCTCTT14350.048.605724
TCTTCCG16550.042.144547
TCCGATC8700.017.02905710-14
CGTTGGA26850.016.0992761
GCTCTTC46950.015.9281495
CCGATCT10850.014.7151910-14
ACGGGAT20050.014.7043559
AACGGGA26950.013.8743348
CGCTATA3900.001854304812.9310211
AAACGGG32400.012.6502017
GAAACGG33900.012.5146846
GGAAACG35950.011.2009965
CCCGAAT14701.2732926E-1110.2920371
CCGAATA10055.1403003E-710.0168122
GATTTCG15906.730261E-119.4970725
Produced by FastQC (version 0.11.5)