FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12320680
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT16900.0114.7441941
GACGCTC26000.074.81262
TTCCGAT28450.069.3378759
ACGCTCT30950.062.8221633
CTTCCGA34450.056.4256748
CGCTCTT37000.052.355264
TCTTCCG39650.050.1150477
TCCGATC22850.018.14840710-14
CGTTGGA69700.016.817631
GCTCTTC121150.016.7029295
CCGATCT27000.015.62557810-14
AAACGGG76500.015.1518847
ACGGGAT45600.014.9989559
GAAACGG81350.014.4291186
AACGGGA64400.014.3095618
GGAAACG84850.013.4944455
AACGGGT18800.011.1056098
CCCGTTG11151.862536E-79.7341711
CTCTTCC219500.09.7110676
GTTGGAA174500.08.2156512
Produced by FastQC (version 0.11.5)