FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936986
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT5000.0128.198331
GACGCTC7350.087.074622
TTCCGAT9050.070.7216649
ACGCTCT10050.064.3969653
CTTCCGA10350.061.8356068
TCTTCCG11050.057.9184237
CGCTCTT11600.055.1722874
CGTTGGA20850.020.034771
TCCGATC7400.018.2706410-14
GCTCTTC37550.018.1929285
ACGGGAT15150.017.5630579
CCGATCT8500.016.24463310-14
AACGGGA21100.016.0178578
AAACGGG25250.015.6635537
GGAAACG28800.013.2334285
GAAACGG28550.012.845566
CGAACTT4650.00700448810.8251533
CTCTTCC71000.010.3307256
CCCGAGT28750.09.7698791
CCGAGTA21650.09.3001322
Produced by FastQC (version 0.11.5)