FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936986
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22150.020.8272881
ACGGGAT13000.019.9360549
AACGGGA20700.019.8236948
GAAACGG24350.018.626096
AAACGGG24050.018.5590927
GGAAACG26650.016.7484475
CCGAGTA16350.011.0078462
GTTGGAA52100.010.5016122
GCGTCGG7550.010.491598140-144
ACGGGAA14551.0913936E-1110.3904999
CCCGACT6355.719005E-410.2163381
CCCGAGT22800.010.1167641
CGAGTAG17300.09.9872353
AAGCGGC6608.2497776E-49.8169973
CCCAAGT43450.08.95841
CCCAAAA62250.07.9898061
CCCAAAG108800.07.35394861
AAAGTGC88700.07.14231734
CAAAGCG14355.96326E-57.03238441
CCAAAGT97600.06.933592
Produced by FastQC (version 0.11.5)