FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608850
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT15350.0122.093461
GACGCTC23300.081.012822
TTCCGAT26900.069.8540349
ACGCTCT29550.063.8522263
CTTCCGA30700.060.9730958
CGCTCTT34300.055.0097164
TCTTCCG33900.055.005157
CGTTGGA59300.020.5000821
TCCGATC20850.019.26776910-14
GCTCTTC106200.018.5805385
CCGATCT23350.017.69816610-14
AACGGGA57200.017.3694618
ACGGGAT41450.016.674399
AAACGGG69000.015.8598037
GGAAACG72450.015.5066665
GAAACGG72950.015.49910456
CTCTTCC193400.010.2774386
ACGGGAA32250.09.8225959
GTTGGAA147700.09.3166812
CGATCTA8350.08.96706110-14
Produced by FastQC (version 0.11.5)