FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596467
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT3800.0125.113221
GACGCTC7950.059.6939052
TTCCGAT8350.056.8374069
ACGCTCT9300.051.801823
CTTCCGA9750.048.674028
TCTTCCG12200.038.3099377
CGCTCTT13000.036.5051164
ACGGGAT17650.023.2223919
CGTTGGA25550.022.554951
AACGGGA24350.020.966088
GAAACGG31050.018.757576
GGAAACG31300.018.3780255
AAACGGG29200.018.2224487
TCCGATC6500.015.93224410-14
CCGATCT8000.013.30453110-14
GCTCTTC42300.011.8990115
GTTGGAA64100.010.7687762
CCGATCG1150.0029476310.004037140-144
CGTTAAT5850.00334830239.85092451
ACGGGAA14952.1227606E-99.1388019
Produced by FastQC (version 0.11.5)