FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4596467
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23600.017.7159231
AAACGGG30400.014.4457997
GAAACGG30400.014.4457996
ACGGGAT17100.014.3142339
AACGGGA25350.014.1996398
GGAAACG30750.013.1107695
AACGGGT8255.3481835E-610.4715888
GCGTCGG8700.09.933196140-144
CCCCGAA6800.0010716239.5407261
CCACGAT7652.9923927E-49.4229391
ACGGGTT7803.682945E-49.2297669
CGCCTAT7150.00169431799.0736981
CCACGAA9101.753566E-48.7136311
CCTCGAA7450.00246151378.7083141
CCTCGGT8306.760531E-48.68499851
GTTGGAA62750.08.4899142
CCCGGGT18751.8553692E-108.4580311
CCCGAGT27350.08.4341341
ACGGGAA15403.3342076E-88.4146699
CGCGACA1550.0035506138.363111140-144
Produced by FastQC (version 0.11.5)