FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11684708
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA27000.052.5339
TCTTCCG32150.044.342438
CGTGTGC42900.035.1244961
CGTTGGA63100.017.1389031
CGACGCT2550.001652799116.9641531
GTGCTCT98150.015.6251644
TCCGATC20000.015.55192710-14
GTGTGCT101450.015.3349362
CCGATCT21550.015.10155310-14
AAACGGG73350.014.331387
AACGGGA61400.014.3062828
ACGGGAT45900.014.2744189
GCTCTTC109400.014.156
GAAACGG76450.014.0327926
TGTGCTC108050.013.993613
GGAAACG79150.013.0992625
TTCCGAT23550.012.53497210-14
TGCTCTT169450.09.6028955
AACGGGT16952.3646862E-119.3451938
CGTATAA24250.08.6219861
Produced by FastQC (version 0.11.5)