FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141754
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24700.021.294481
ACGGGAT17250.016.6940369
AACGGGA25300.016.2197728
AAACGGG30300.015.444057
GAAACGG29850.015.4356916
GGAAACG31300.014.720625
GCGTCGG13550.013.073397140-144
CAAAGCG18150.09.9244051
AAGCGCG5000.09.793356140-144
GTTGGAA67900.09.5425222
TATCCAC53500.09.1505166
GCGGGAT6600.0090791348.7264289
ACTTCGT8458.182532E-48.5198856
AAGCGGC7950.00447923278.1501543
CCCAAAG143400.07.93869351
CGAGAAA11051.6526246E-47.82456251
CGCGTCG2951.3178924E-67.8108864135-139
ATCCACT60750.07.70295767
CCACTTG74850.07.69464549
CCCAAAT79650.07.6890781
Produced by FastQC (version 0.11.5)