FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17565336
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT84850.0135.09621
GACGCTC98150.0116.129492
ACGCTCT109850.0103.884673
TTCCGAT115100.099.2087869
CTTCCGA121600.093.668848
CGCTCTT120900.092.781934
TCTTCCG133900.085.279557
GCTCTTC232850.049.5655025
CTCTTCC383150.030.3665056
CCGATCT115800.022.51967810-14
TCCGATC112750.021.08544310-14
CGACGTC4003.839159E-718.0008071
CGATCTA29400.016.89756210-14
CGTTGGA97250.016.6588441
AAACGGG111250.014.3025857
GAAACGG118750.013.4598936
AACGGGA93550.013.1605668
CGATCTT74650.012.78898210-14
CGATCTG37150.012.75232410-14
ACGGGAT68600.012.6994099
Produced by FastQC (version 0.11.5)