FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11202731
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT4600.034.9325521
CGGCGTA2350.034.657651
CGGTATA12650.034.1060031
CGGTATG12000.032.8350031
CGGACCA9950.031.6357571
GCGGGTA7900.031.4859391
CGGTACA14450.031.3808481
GCGCATT13350.031.1634711
CGGTAAG10400.030.9018731
TACGGCG1000.030.7991896
CGCCGAT1650.030.68381
CGGTATT19500.030.5914421
CGGTATC9550.030.425371
CGGCGAT4200.030.3979281
CGGCGTT4600.030.147271
GCGTACT6800.030.105051
GCGTACC6000.030.0834661
GCGTGTA13250.030.069621
CGGTTAG10400.030.0552461
CGGTGTA14650.029.9006561
Produced by FastQC (version 0.11.5)