FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11202731
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA259620.23174706239041173No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG42400.014.4747931
TAGCCGG44950.014.3877092
TACTCGG32800.013.346065538
CTACTCG34650.013.3318437
GGCGCCT25150.013.21416820
ACTCGGG32750.012.6947639
CTCGGGA32000.012.57995140
GCGCCTG34700.012.36818621
CGGTCGT1300.003581281311.8448261
TCGGGAG34850.011.61429741
CGGGCGT22100.011.5462176
GGTGGCG49200.011.44587113
AGCCGGG55800.011.4324113
TGGCGGG34200.011.32036915
GGGCGCC24450.011.16204219
GTAGTCC99800.010.86680827
GCTACTC98450.010.85926336
TAGTCCC105900.010.71863328
CAGCTAC164750.010.52157134
TGTAGTC111650.010.48186426
Produced by FastQC (version 0.11.5)