FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8994151
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACC5450.034.3221051
CGGTATA11700.034.2323761
CGGTACA10400.033.6445921
CGGTATT19250.032.924051
GCGCGTA2150.032.75391
CGGTGTA12400.032.477371
CGGTACT10350.032.106141
GCGTACT6800.032.0389141
GCGTATC7300.031.9546811
GCGTATT14150.031.726721
GCGTATA11200.031.4378951
TACGGCG1400.031.4282846
GCGCGTT3450.031.2556441
CGGTAAT13950.031.0773181
GCGTGTT15250.030.8812891
CGGTATG10650.030.7884711
CGGCGTA1950.030.4705731
CGGCGAT3650.030.1459261
CGGTAAC6450.030.0244051
CGGCGGA2650.029.8956581
Produced by FastQC (version 0.11.5)