FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8994151
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153650.170833244849903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG27200.012.1313982
GGCGCCT17700.011.06533620
TTAGCCG26150.010.5995471
GCGCCTG24950.010.05500221
CGGGCGT13350.010.0516426
TGGCGGG23950.09.82803615
GGTGGCG30850.09.626471513
GTAGTCC61550.09.61435127
GTGGCGG24700.09.35149114
TGTAGTC69450.09.18589526
CTACTCG25150.09.18416837
CGGCAAT2651.05436695E-49.1313561
GGGCGCC16150.09.12956619
TCGTATA5601.2732926E-119.03500411
TAGTCCC65250.09.0017428
AGCCGGG37000.08.9776723
TACTCGG25250.08.88642938
CCTGTAG72900.08.87187824
TAGTCGG2250.00224040248.7993082
AGCTACT112450.08.54890435
Produced by FastQC (version 0.11.5)