FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8890571
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAT3500.036.4753761
CGGTATA12250.034.1395151
CGGTATT18400.032.896881
CGGTACA12250.032.8817441
CGGTATG10700.032.5022281
CGGTGTA11800.031.8973031
GCGCGTA1400.031.444291
CGGTACT9650.031.2487811
CCGTATA6150.031.1375161
CGGTAAT11500.031.0068051
CGGCAAT11450.030.7577321
GCGCACT9450.030.0467661
CGGTGTT18400.029.9062561
CGGTATC8500.029.7795941
GCGTACG1700.029.7795931
GCGTATT12300.029.7059061
TACGGCG2000.029.6993146
GCGCATT12700.029.4635471
CGGTCAT10550.029.417551
GCGCCTT12800.029.4053231
Produced by FastQC (version 0.11.5)