FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8890571
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA146180.16442138530809777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG27700.012.4678312
TTAGCCG26700.012.1933051
GGCGCCT17450.011.09885320
CGGGCGT12950.011.0411576
GCGCCTG22650.010.29979321
GTAGTCC59100.09.67801227
AGCCGGG35800.09.6468963
TGGCGGG21950.09.52050715
GGTGGCG31150.09.46275813
GGCTCGA4002.1649612E-89.34951931
GTGGCGG22600.09.34402114
TACTCGG24250.09.16179738
CTACTCG24500.09.15809437
GGGCGCC16250.09.07428319
TGTAGTC66200.09.07204126
TAGTCCC63800.08.7581728
GCTCGAA4551.8813807E-88.70284832
CTTCGTA5601.200533E-108.6418299
CTGTAGT73050.08.61283625
CCTGTAG72750.08.46691824
Produced by FastQC (version 0.11.5)