FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10744021
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1900.038.2214851
CGGTATA13800.033.8067971
GCGCCTT26150.033.5775071
CGGTCTT24450.032.941961
CGGTCTG14250.032.7392121
GCGCGTT2450.032.3358041
GCGCCCT22400.032.3217661
CGGTCAT11600.032.250631
CGGTACA12800.032.1498451
CGGTCCT22550.032.106771
CGGTATC9050.031.6112751
GCGACTT8800.031.5090351
GCGTACT7250.031.2641371
CGGTATT19200.030.9463731
CGGACCA10900.030.8895931
GCGCCTA12850.030.8259621
CGGTATG11500.030.6174761
CGGTGTA11000.030.6087781
GCGTCCA12650.029.9216231
GGCCCTT118500.029.9174421
Produced by FastQC (version 0.11.5)