FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10744021
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1785531.6618824553675016No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA358560.3337298019056366No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326170.30358280200680915No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240710.22404088748523482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT19800.015.11086220
TTAGCCG34300.014.1833391
CCTAGTC20700.014.13503216
TACTCGG28600.013.99976938
TAGCCGG37500.013.9624362
CTACTCG29900.013.611814537
GCGCCTG28450.013.532289521
ACTCGGG30950.013.15008739
GGGCGCC18550.012.80841419
GGGTTGC23600.012.7709766
CTCGGGA27800.012.74085640
GCTACTC83550.012.40194936
CTAGTCT23700.012.25296217
CGACTAG1455.835988E-412.1451361
AGCCGGG45900.012.0303023
TCCTAGT25400.011.77933315
GTAGTCC80150.011.77533627
CGGGCGT17450.011.7247356
AGCTACT138050.011.64921135
GGTGGCG36450.011.64864313
Produced by FastQC (version 0.11.5)