FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12331181
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1450.034.9048271
CGGCGTA2450.034.1305431
GCGCCTT25000.033.6239741
CGGTATA16800.032.352911
CGGCGAT3850.032.007591
CGGTCTT25150.031.323531
CGGTACG1550.031.2332131
CGGTCCT19300.030.7845021
GCGCCCT22750.030.7589421
CGGTATG14000.030.4929451
GCGTACA9700.030.3989621
GGGCGTA6450.030.3637891
CGGTACT9800.030.3133091
CGGTGTA13000.030.2994941
GGCCCAT113000.030.2620411
GCGCATT14550.030.2477231
GGGCGAT9400.030.198651
GGGCGTT11450.030.1730941
CGGTACA13000.030.1302221
GCGACCA12300.030.0559061
Produced by FastQC (version 0.11.5)