FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12331181
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2515272.039764074503488No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA684110.5547806004956054No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590030.478486205011507No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469770.38096107745073243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG44700.017.2345491
GGCGCCT29600.016.8713520
TAGCCGG49850.016.7702452
GCGCCTG38050.015.43733221
TACTCGG35950.015.29884138
CTACTCG37500.015.01848637
CGGGCGT22950.014.8581486
ACTCGGG36200.014.8285539
GGGCGCC27050.014.3140619
CCTAGTC25250.014.11468116
CTCGGGA33900.014.08242640
AGCCGGG61650.013.9170513
GCGGGCG21150.013.83434917
GGTGGCG55100.013.73486113
ACGCGCC2256.7393557E-913.68872419
TGGCGGG40050.013.56787615
GGGTTGC28950.012.8426256
TCGGGAG37900.012.77029541
GTGGCGG42900.012.76907714
GCTACTC107700.012.76673936
Produced by FastQC (version 0.11.5)