FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9537104
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1900.034.747421
GCGCCTT21100.033.687981
GCGCGTA1250.033.4501841
GCGACCA10200.032.147041
CGGTATA12100.032.0097431
CGGTATC7500.031.9830721
CGGTCTT19850.031.818251
CGGTATG10450.031.7991561
GCGACCG2400.031.1761571
CGGTCCT17150.031.0531861
GCGTACT6950.031.0310291
GCGCCCT17700.030.8342481
CGGACCA9650.030.5585251
CGGTACT8100.030.4290181
CGGTATT18100.030.3959961
GCGTATT9500.030.3460831
GCGCATT9800.030.3153511
CGGCGAT2700.030.1573281
CGGTACA10850.030.0183541
GCGTCTA7800.029.6244031
Produced by FastQC (version 0.11.5)