FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10334667
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTT2750.037.6067851
CGGTATG11300.033.6874921
GCGCCTT20250.033.685091
CGGTATA12350.033.3177531
GCGCCTA11550.033.1488381
GCGACCA10800.033.0059551
CGGACCA10850.032.8538551
CGGCGTA2350.032.771871
GCGCATT11750.031.8355311
CGGTCCT17600.031.6307071
GCGACTT9200.031.5709131
CGGTATC10400.030.6786121
CGGTCTT21750.030.6538071
CGGTGTA10850.030.6230371
GGCCCAT99900.030.3297961
CGGTCTA10900.030.0788211
GCGTACC3750.029.92541
CGGTGTC12500.029.9253981
GGCCCTT120850.029.8969961
GCGTCCT17600.029.880391
Produced by FastQC (version 0.11.5)