FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10334667
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1511451.462504790913921No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374760.362624165829436No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335480.3246161680874672No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA252660.24447812396858068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG31400.015.90418237
GGCGCCT25500.015.78796120
TACTCGG30400.015.63130738
TTAGCCG35900.014.9632441
GCGCCTG33500.014.77586321
TAGCCGG39200.014.7600192
CCTAGTC20850.014.34987116
ACTCGGG31300.014.33840939
CTCGGGA30550.014.0423140
GGGCGCC22550.013.95097819
CGCGCCT4600.013.86932420
TGGCGGG31450.013.29070715
GGTGGCG47250.012.9436913
CGAATCT4600.012.91288235
GCTACTC91350.012.86020436
TCGAATC4850.012.70087134
TCGGGAG34000.012.68213341
AGCCGGG48450.012.4868833
GTGGCGG33900.012.4599614
GCGGGCG18300.012.38229917
Produced by FastQC (version 0.11.5)