FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10072250
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1377421.3675395269180173No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357860.3552930080170766No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317160.3148849561915163No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239910.23818908386904614No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCCT22950.016.6795320
TACTCGG28800.016.57618138
TTAGCCG35950.016.4703771
TAGCCGG39050.016.168812
CTACTCG30750.015.81118437
ACTCGGG30150.015.32319239
GCGCCTG31500.014.94584621
CTCGGGA28600.014.69212440
GGTGGCG43000.013.55797113
GCTACTC90350.013.51394336
TGGCGGG30900.013.38494615
CCTAGTC18850.013.30487316
GGGCGCC21550.013.16920919
GCGTGGT60700.013.0838579
GTAGTCC85550.013.06354227
CAGCTAC148350.012.886929534
GGCTCGA4300.012.79053831
AGCCGGG50400.012.7895223
TAGTCCC91900.012.639667528
GGCGTGG64750.012.6392248
Produced by FastQC (version 0.11.5)