FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9560193
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1300.037.235641
TATCGCG1050.035.6186838
GCGACCA10850.033.663391
CGGTCCT15150.032.6775441
TCGCGTA1150.032.52140440
GCGCCTT22400.032.5131841
GCGTACT6250.032.3882371
CGGTCCA10400.032.1580541
CGGTCTT20250.031.6189541
CGGTATC8850.031.3261361
GCGCCCT16500.031.0707321
CGGCGTA1350.030.9670141
CGGACTG10050.030.869711
CGGTACA10500.030.804031
CGGTATA13250.030.7210031
CGGTGTA11400.030.6882271
CGGTACT8350.030.5668751
GGCCCTT100150.030.5162261
GCGCGAA651.7475031E-830.4655251
GCGCCTA12000.030.4373171
Produced by FastQC (version 0.11.5)