FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9560193
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1600701.6743385829135458No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366620.3834859819252603No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325270.3402337170389761No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247630.2590219674435443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG112090.11724658696743884No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGG38400.016.0987552
GGCGCCT21500.016.06482920
TTAGCCG34900.015.8344071
GCGCCTG27950.015.11243221
TACTCGG25650.014.75217338
CTACTCG26250.014.24736537
ACTCGGG26350.014.10980639
TGGCGGG27150.014.099195515
GGGCGCC19850.013.96448619
GGTGGCG40400.013.88589113
AGCCGGG46600.013.8795813
CGGGCGT18300.013.4642416
CCTAGTC18250.013.38058516
GCTACTC77250.013.32791836
CTCGGGA24600.013.05667840
TAGTCCC83900.012.95328528
GTAGTCC81050.012.9201927
CAGCTAC131600.012.72165834
GTGGCGG29350.012.66757614
GCTCGAA4000.012.64977532
Produced by FastQC (version 0.11.5)