FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9381173
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTA1950.036.1132431
GCGCCTT20950.034.2440151
TATCGCG1100.033.9988238
CGGTATA12250.033.2343181
CGGTACG1200.033.009761
GCGACCA9200.032.531361
TCGCGTA1150.032.5206140
CGACCTA3750.032.2734572
CGGACCG2050.032.2046431
CGGTCTT18700.031.5387361
CGGACCA8550.031.4010981
CGGTCCT15500.031.2350431
CGGTATG10800.031.1758841
CGGCGTT3900.031.0348191
CGGTACT7450.031.0158821
GCGTCCT14150.030.9490781
CGGTACA11500.030.809111
CGGTGTA9450.030.5063761
CGGCGAT2600.030.4705491
GGCCCAT86150.030.3723011
Produced by FastQC (version 0.11.5)